Page 133 - congress

ORAL PRESENTATION / SÖZLÜ SUNUM

Antioxidant and Antiproliferative Effects of Propofol, Ketamine, and
Their Combination Against Hydrogen Peroxide-Induced Oxidative Stress in A549 Cells

Zeyno NUHOĞLU¹ * Abdurrahman AKSOY 1
,

¹Department of Veterinary Pharmacology and Toxicology, Faculty of Veterinary Medicine,
Ondokuz Mayıs University, Samsun, TÜRKIYE

*Correspound Author: zeyno.nuhoglu@omu.edu.tr

This study aims to investigate the antiproliferative and antioxidant effects of ketamine,

propofol, and their combination in A549 lung cancer cells under oxidative stress induced by

hydrogen peroxide (H₂O₂).
Initially, the cytotoxic effects of propofol, ketamine and their combinations (10–2000

µg/mL) were evaluated over 24-hours using the MTT assay following exposure to 100-2000
µM H₂O₂. Based on the results, the IC₅₀ value of H₂O₂ was determined to be 501 µM. For

propofol, ketamine, and the propofol-ketamine combination, the three most effective
concentrations (75, 125, and 250 µg/mL) were identified and used for subsequent

experimental treatments. Oxidative stress markers, including malondialdehyde (MDA), and

antioxidant parameters such as superoxide dismutase (SOD), total glutathione (tGSH), and
total antioxidant capacity (TAS) were measured in cell lysates following treatment with the

selected doses.
Both propofol and ketamine were found to suppress H₂O₂-induced cell proliferation and

reduce oxidative stress. Treatments with increasing concentrations significantly decreased
MDA levels while enhancing SOD, tGSH, and TAS levels. Notably, the combination therapy

demonstrated a stronger antioxidant effect. compared to individual treatments, suggesting

an additive or potentially synergistic interaction.
These findings suggest that ketamine and propofol may exert antiproliferative and

cytoprotective effects in lung cancer cells not only through their anesthetic properties but
also via modulation of oxidative stress. The observed reduction in reactive oxygen species

(ROS) and cytotoxicity supports the potential application of these agents as adjunctive
therapies in lung cancer treatment. Further molecular and in vivo studies are warranted to

elucidate the underlying mechanisms and assess their clinical relevance.
Keywords: A549 cells, anesthetics, cytotoxicity, oxidative stress, ketofol.

130